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ROCK Activity Assay Kit (ab211175) provides a non-isotopic sensitive and specific method to monitor ROCK1 (ROKβ) and ROCK2 (Rho Kinase) using its physiological substrate MYPT1 (myosin phosphate target subunit 1) This kit can also be used in the screening of Rho kinase inhibitor molecules in
Cell Biolabs' Checkpoint Kinase Activity Immunoblot Kit utilizes recombinant Cdc25C as checkpoint kinase substrate After incubating the substrate with checkpoint kinase samples (such as purified kinase cell lysate or immunoprecipitate) the phosphorylated Cdc25C is detected by western blot analysis using an anti-phospho-Cdc25C (Ser216)
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NOTE For IP kinase assays ~15 L of unsuspended beads is a typical starting sample For recombinant protein kinase assays typical starting amounts range from 0 1-1 0 g in 1-10 for 25-50 L final reaction volumes Adjust the volumes of recombinant protein samples to be equal with the buffer they are stored in prior to initializing the assay
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Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit If you are also studying Rac1 or RhoA you may consider one of our economical combination kits
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